Our work is being led by Giovanni Ghirlanda, the principal investigator and Melissa Ruben, grad student.
Monday we were shown the location of most of the lab work for this project, given the first set of papers regarding the project to read and met Melissa.
Tuesday we were shown the correct concentration of material and liquids required to produce the medium required for the growth of the e. coli virus. In doing this, correct sterility, non-contamination lab skills and lab safety (i.e. gloves, close-toed shoes, googles and common lab sense) were covered. We subsequently mixed both medium and agar solution and adjusted pH to 7.5 before we autoclaved both mixtures. Proper use of the autoclave, measuring instruments, sterility of lab equipment (using bleach for E. coli containers), use of the pipette calibration unit, and the optical density calibrator were shown.
Wednesday we were given the second paper on their lab project to read and study. Melissa had prepared petri dishes with E. coli cultures the afternoon before and we examined the number of phage circles present on each.
Thursday we met Giovanna Ghirlanda and Melissa took us to the chemistry computer lab to show us the chemistry program available to the chemistry department here at ASU. Great stuff!
Friday Giovanna gave us the short version of the protein binding project and clarified a few grey areas for us. Melissa showed us how to use the DNA concentrator and prepared a new mixture for electrophoresis.
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